Olympus IX71 - Manual (Page 41)

37
IX71/IX51
1. Attach the phase contrast slider @ to the illumination column so that the
slider’s indication surface faces upwards and the finger hook position
comes on the right.
2. Be sure to set the aperture iris diaphragm lever ² to the open position
( ) for phase contrast observation.
}The IX2-SLPH2 optical element does not need centering.
}Before proceeding to the following, open the aperture iris diaphragm
because flare would be observed at the center when it is stopped down.
1. Engage the phase contrast objective in the light path and bring the
specimen into focus.
2. When the U-BI90CT binocular observation tube is used, rotate the CT
turret @ to position “CT”. When the observation tube in use is other than
the U-BI90CT, remove an eyepiece and attach the U-CT30 centering
telescope in place. (Fig. 56)
3. Engage the optical element matching the phase contrast objective in
the light path.
4. Rotate the focus ring ² (or the knurled section when the U-CT30 is used)
to focus on the ring slit ³ and the phase plate | of the objective.
(Figs. 56 & 57)
5. Using the optical element centering knobs …, turn the two centering
screws ƒ of the phase contrast slider so that the ring slit image overlaps
with the phase plate of the objective.
}A ghost of the ring slit image may be observed. In this case, overlap the
brightest image with the phase plate.
}If a thick specimen is moved, the ring slit image may be deviated from
the phase plate and the contrast may be deteriorated. In this case,
re-adjust the centering by repeating steps 1 to 5 above.
6. After completing centering, rotate the CT turret to return the turret to
position “0”. If the centering telescope is in use, replace it with the
eyepiece.
}If the vessel is not completely flat, it may become necessary to adjust the
centering again to obtain the optimum contrast.
Repeat centering by beginning with the lowest-power objective and
increasing the objective power in order.
}Engaging the green filter in the light path will improve the contrast.
Fig. 55
@
²
Fig. 56
Fig. 57
Fig. 58
ƒ
…
3 Attaching the Phase Contrast Slider (Fig. 55)
4 Centering the Phase Contrast Ring Slit (Figs. 56 to 58)
@
²
³
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