Hoefer SE300 miniVE - Manual (Page 31)

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p23
Important! Buffer conductivity
increases with increasing
temperature, providing a
positive feedback that results in
rapid heating. We recommend
programming the power supply to
hold the current setting constant
to avoid possible overheating,
especially if no passive cooling is
in place. If the only programming
option is to hold the voltage
setting constant, monitor and
adjust the voltage to maintain the
current at or below 400 mA.
Electrotransfer
Electrophoretic transfer conditions for blotting
proteins in Towbin buffer: 25 V for 1–2 hours,
300 – 400 mA.
After electrotransfer

Turn off the power supply and disconnect the leads.

Remove the safety lid.

Lift out each module and drain it by inverting it over a
sink. Avoid wetting the banana plugs with buffer.

Open the module. Remove the gels and membranes.
Save the packing sponges. Discard the blotting paper.

Label each membrane and indicate the sample side.
Lift the membrane(s) with blunt forceps and allow to
air dry.

Rinse the unit immediately after use.
Blotter care and maintenance
• Do not autoclave or heat any part above 75 °C.
• Do not use organic solvents, strong or oxidizing
cleaning solutions, abrasives, or strong acids or
bases on any part of the instrument.
• Immediately after each use, rinse the unit with
water and then rinse thoroughly with distilled water.
Handle the module with care to prevent damage to
the electrode plugs. Allow to air dry.