Hoefer SE300 miniVE - Manual (Page 30)

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p22

Check the position of the transfer stack.
The transfer stack should be centered on the elec-
trode plate. No layer should be pinched when the
module is closed.

Fold the empty half of the cup over the stack and
press the halves together to snap the module closed.
The transfer stock should be held firmly in place
when the cup is closed. Replace old and compressed
sponges, if needed, to fill the cup.
Final assembly

Slowly pour 300 – 350 ml of transfer buffer into the
top of the module, to allow air to be displaced by the
buffer as it fills the cup. Tap the blotting cup lightly
to dislodge any air bubbles in the packing sponges.

Position the module(s) in the tank with the banana
plugs toward the center, the red side facing outward.

Add deionized water to the tank — 1.7 liters for one
module and 1.2 liters for two modules.
To avoid rapid evaporation, buffer temperature should
not exceed 75 °C. Passive cooling is recommended if
the transfer will be longer than one hour, if biological
activity must be retained, or if transferring nucleic
acids. Chill deionized water to ~4 °C before adding to
the tank.

Place the safety lid on the tank. Either orientation fits
and is correct.
Plug the color-coded leads into the jacks of an
approved power supply, such as the PS300B or
PS200HC: red to red, black to black.
Fig 16. Final assembly.
red anode side (+) faces
the outside tank wall
black
cathode
side (–)
faces the
center