Hoefer SE300 miniVE - Manual (Page 21)

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p13
Note: The amount of protein
sample added to each well
depends on both the sensitivity of
the staining method and the distri-
bution of protein among separate
bands. With Coomassie
™ Blue,
it is possible to detect 1 µg in a
single band; with the more sensi-
tive silver stains, it is possible to
detect as little as 10 ng.
Underlay the sample into the wells using a micro-
pipet or fine-tipped microsyringe. Table 1 shows the
volume of sample required for different numbers of
wells and comb thicknesses.
Table 1:
Well capacities: volume of sample (µl) per 1mm depth
comb thickness (mm)
no. of wells 0.75 1.0 1.5
5 9.5 12.7 19.1
9 — 5.8 —
10 3.6 4.8 7.2
12 — 4.75 —
15 2.2 2.9 4.4
18 — 2.9 —
Electrical connections

Position the safety lid over the unit and seat the lid so
the banana plugs engage the jacks in the lid. The lid
is symmetrical and fits in either orientation (Fig 13).

Plug the color-coded leads into the jacks of an
approved power supply (red to red, black to black).
The minimum power supply rating is 250 V, 50 mA,
constant current or constant voltage. (Recommended
power supply: PS300B.)
Fig 13. Fully assembled miniVE
with electrophoresis module.