Hoefer SE300 miniVE - Manual (Page 19)

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
Check that both gel surfaces will contact buffer.
Check that the bottom gel-contact slot is exposed.

Move the sealing plate into the “half open” position to
prepare for electrophoresis. Apply gentle inward pres-
sure to both tabs and lock them into the lower notch.
Final assembly

Make sure the sealing plate is in the “half-open” posi-
tion. The arrow in Fig 12 indicates the correct position.

Lower each module into the tank, seating it in the
locating slots.
The module seats properly in only one orientation —
with the banana plugs toward the center of the tank
and the gel facing outward.

Add the appropriate amount of electrophoresis buffer
to the tank.
Add 1.2 – 1.6 liters of buffer to the tank when only
one module is in place, and 1.1 – 1.4 liters when two
modules are in place.
Fig 11. Securing the cassette.
a
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