Hoefer SE300 miniVE - Manual (Page 18)

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
Insert a comb (at a slight angle to prevent trapping
air) into the sandwich, allowing the comb sides to rest
on the spacers.

Allow a minimum of one hour for the gel to polymerize.
Working with precast gels

Prepare the electrophoresis module as described in
“Preparing the module” on page 4. Follow the manu-
facturer’s instructions to prepare the gel for electro-
phoresis. This may involve removing tape or breaking
off the sealing edge from the bottom of the cassette.

Remove the comb and rinse the wells with electropho-
resis buffer to remove any unpolymerized acrylamide.

If the gel is ready for electrophoresis, move the
sealing plate into the “half open” position. Apply
gentle pressure to both tabs and lock them into the
lower notch.

Position the cassette on the module. Orient the
cassette so that the notched side is against the gasket,
and the wells are at the top of the module. Center the
cassette within the guiderail at both sides of the module
(a) (Fig 11).

Secure the cassette. Swing each clamp into position
over the sides of the cassette. Tighten each screw,
alternating to apply even pressure until the cassette is
secure. The gasket around the upper buffer chamber
should be fully compressed to provide a seal, but the
screws should not be tightened to the point that pres-
sure stresses the cassette.
Note: If the gel has wells, skip to
“Final assembly” on page 11.
Tip: To calculate the volume,
measure the distance in centime-
ters, from the top of the resolving
gel to the notch in the glass plate.
This should be at least 2 cm.
Multiply this distance by the gel
width (8 cm) and the gel thickness
(cm) for the required volume (ml).
Note: To aid in sample loading,
mark the well locations with a
laboratory marking pen.