Hoefer SE300 miniVE - Manual (Page 22)

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Electrophoresis
For optimal resolution, start electrophoresis
immediately after sample loading.
Gels may be run at either constant current or
constant voltage. For Laemmli SDS separations,
the recommended voltage range is 100 –250 V
and should not exceed 300 V. If running gels
at constant current, the current should be
10 –20 mA per gel, depending on gel thickness
(10 mA for 0.75 mm, 15 mA for 1.5 mm).
Check progress after 5 minutes, and again after
half an hour, monitoring the position of the
tracking dye. The run is complete when the
tracking dye reaches the bottom of the gel.
After electrophoresis

Turn off the power supply and disconnect the leads.

Remove the safety lid and lift out the module(s).

Release each gel sandwich or cassette from the module.
Move the sealing plate to the fully open position by
pressing inward on both tabs and guiding the plate to
open out. Then loosen all four screws 4 – 5 turns in the
counterclockwise direction. Swing the clamps outward.

Remove the gel from the sandwich or cassette.
Gently loosen and then slide away both spacers. Slip
an extra spacer or the Hoefer Wonder Wedge into the
bottom edge to prevent breaking the “ears” of the
notched plates and separate the plates.
If using precast gels, follow gel manufacturer’s
instructions.