Hoefer SE300 miniVE - Manual (Page 20)

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The minimum and maximum levels are marked. Verify
that the lower electrode, which is approximately
2 cm from the bottom of the module, is completely
submerged. To prevent buffer from entering the upper
buffer chamber, verify that the buffer level is not
above the maximum level.

Add the appropriate amount of electrophoresis buffer
to the upper buffer chamber.
Fill the upper buffer chamber to a level 3 – 5 mm
above the notched plate. This requires approximately
100 ml.

Prepare and apply the sample.
Increase liquid sample density with 10% glycerol or
sucrose. Add a tracking dye, such as bromophenol blue.
Fig 12. Preparing for
electrophoresis.
Tip: As an aid in loading samples,
mark the well location with a labo-
ratory marking pen or use locating
decal. The locating decal only
works with miniVE combs and not
precast gels.
the half-open postion
for electrophoresis
upper buffer chamber