beautypg.com

7 photometry (lcs iv only) – ALTANA LCM IV User Manual

Page 70

background image

71

Standard programs

5.7 Photometry (LCS IV only)

In the photometry mode, photometric measurements can be

carried out as:

Single wavelength measurements

Multi wavelength measurements

Time course

Wavelength scans

5.7.1 Single Wavelength (absorbance, concentration and transmittance readings)

The Single Wavelength mode can be used in three ways. For

sample readings at a single wavelength, the instrument can be

programmed to measure the absorbance, % transmittance or

concentration of the analyte.

Absorbance readings: The light absorbed by the sample is

measured in absorbance units.

% Transmittance measures the percent of the original light

that passes through the sample and reaches the detector.

Turning on the concentration factor allows selection of a

specific multiplier for converting absorbance readings to

concentration. In a graph of concentration versus the

absorbance, the concentration factor is the slope of the line.

5.7.1.1 Set up Single Wavelength mode

1. In the main menu, press the Single Wavelength menu

option.

2. Press Options for Parameter Setup.

Table 7 Options for Single Wavelength

Options

Description

More

For further Options

Save icon

Symbol:

Store Data, if Instrument Setup > Data Log Setup > Auto Store: Off is selected.

Symbol:

Recall Data, if Instrument Setup > Data Log Setup > Auto Store: On is selected.

% Trans/Abs

To

switch to % transmittance, concentration or absorbance readings

This manual is related to the following products: