BUCHI ELS Detector C-650 - Manual (Page 24)

C-650 Operation Manual, Version B 5 Putting into operation 24
Use the large black nut to position the nebulizer on the glass chamber.
Fill the siphon overflow on the nebulizer/glass chamber assembly with the mobile phase that will
be used for the separation. If you are using a very volatile solvent (e.g. hexane or CH
2
Cl
2
), then
use water to fill the overflow. The liquid should fill the bent part of the siphon, but should not pool
in the bottom of the evaporation tube.
Make sure that no liquid leak could affect the detector performance or create laboratory pollu-
tion.
5.5.1 Connecting the siphon overflow
Attach a Tygon drain tube assembly to the end of the siphon tube using the tapered hose con-
nector and lead the tube to waste and drain. Locate the tube in such a way that the discarded
part of the solvent can flow freely from the siphon and ensure that the end of the tube is not im-
mersed in the collected liquid. Make sure that liquid waste container complies with the solvent
nature.
Ensure that no siphon liquid leak could affect detector performances or create laboratory pollu-
tion.
Install the drain tubing (it can be cut) in a way to the siphon outlet aligns straight to the waste con-
tainer without loops or bends, so that the waste liquid flows smoothly through the drain tubing.
Fix the drain tubing at the inlet of the waste container so that the end of the drain tubing never
dives into the liquid in the container.
A drain tube with a bend or immersing the liquid will create pressure fluctuations in the detector
and will lead to poor results.
If the solvent that you are using is not compatible with Tygon (e.g. THF), use a piece of PTFE tub-
ing or any material you know compatible with your solvent in its place.
Please check your local regulatory authorities for recycling solvents and health and safety re-
quirements.
5.5.2 Connecting the nebulization gas to the nebulizer
Attach the nebulization gas tube coming out from the front panel to the nebulizer gas inlet fitting
located on the nebulizer side. Refer to Fig 5.10.
NOTE
Make sure you are using the correct black gas tubing orientation, where the white one-way valve
is at the lower end (near the gas arrival).