BUCHI ELS Detector C-650 - Manual (Page 13)

C-650 Operation Manual, Version B 4 Description of function 13
4 Description of function
This chapter explains the basic principle of the device, shows how it is structured and gives a
functional description of the assemblies.
4.1 Overview over the device
Fig 4.1: Overview over the device
The C-650 is designed to detect compounds in the eluent from Preparative Chromatography. It
is capable of monitoring eluent flow rates from 0.1 mL/min to 5 mL/min. Evaporative Light-Scat-
tering Detection is a nearly universal technique which can detect any non-volatile analyte. Un-
like other types of detection mode such as UV Detection, it is not dependent on the absorption
of radiation and is not affected by the absorption characteristics of the solvent. Thus, solvents
which absorb UV radiation can be used. As the solvent is completely evaporated, a gradient can
be performed to optimize the separation.
The detector is controlled via the keypad and digital LCD display on the front panel or by RS-232.
The analog signal output can be sent to a recorder, an integrator or a data station such as BUCHI
SeparcoreRecord or SepacoreControl Software.
The detector includes a nebulization cell, an evaporation tube and a detection chamber. The
evaporation tube is heated in order to evaporate the solvent.