Dako Autostainer Plus - Manual (Page 75)

Dako Autostainer Plus | User Guide 75
Compatibility Check
A compatibility check has been built into the Dako Autostainer Plus software to minimize programming
errors resulting from assigning incompatible reagents (such as alkaline phosphatase-labeled
streptavidin and DAB substrate) to a slide protocol. This feature has two check levels. The first check
is the species reactivity compatibility of the primary antibody and the secondary reagent used. The
second addresses the compatibility rules dictated by the enzyme used in the detection system. The
following tables summarize the compatibility rules and codes assigned to each reagent type.
Every reagent in the reagent categories lists (Protocol Element) can be programmed with an
appropriate compatibility code or combination of codes. For example a linking reagent that reacts with
mouse and rabbit can be marked with compatibility codes AB. The software verifies that these
compatibility rules are not violated during the programming of a staining run. If an incompatible
reagent is assigned to a tile in the programming grid, an INCOMPATIBLE REAGENTS dialog box
displays the two reagents which are incompatible.
Select the NO button to replace an incompatible reagent. The incompatible reagent is removed from
the last programmed tile and the reagent list corresponding to that tile is displayed.
Select the YES button to override the warning message. The next available tile for programming
flashes and the corresponding reagent list is displayed.
A. Species Reactivity
Reagent Type (Protocol
Element) Description Compatibility
Code
Primary Antibody All monoclonal primary antibodies raised
in mouse (e/b., mouse anti-human A
Primary Antibody All polyclonal primary antibodies raised in
rabbit (e.g., rabbit anti-human B
Secondary Reagent All secondary reagents (antibodies)
compatible with monoclonal antibodies
raised in mouse (e.g., biotinylated anti-
mouse lgG) A
Secondary Reagent All secondary reagents (antibodies
reacting with polyclonal antibodies raised
in rabbit (e.g., biotinylated anti-rabbit lgG) B
Secondary Reagent All secondary reagents (antibodies)
reacting with moth monoclonal and
polyclonal antibodies raised in mouse or
rabbit, respectively. AB
B. Enzyme System Compatibility
Reagent Type (Protocol
Element)
Description Compatibility
Code
Endogenous Enzyme Block, HRP-compatible X
Endogenous Enzyme Block, AP-compatible Y
Tertiary Reagent HRP-labeled streptavidin X
Tertiary Reagent AP-labeled streptavidin Y
Substrate HRP-compatible substrates (i.e., DAB,
AEC)
X
Substrate AP-compatible substrates (i.e., Fast Red,
New Fuchsin) Y