Dako Autostainer Plus - Manual (Page 109)

Dako Autostainer Plus | User Guide 109
Shelf Life and Storage
PBS solutions should be stored at 2-8° C to inhibit bacterial growth. PBS solutions that become
turbid should be discarded.
If phosphate buffered saline is stored at 4° C, the expected shelf life is 7 days.
If phosphate buffered saline is stored at room temperature, the expected shelf life is 4 days.
NOTE: Dako PBS does not contain any preservatives. It is recommended that the wash buffer
carboy is washed according to good laboratory practices each time before it is filled with fresh
buffer. Also, it is recommended that the end piece of the in-line buffer tubing is wiped clean
periodically to avoid clogging.
Phosphate Buffered Saline
The following is a procedure for the formulation of 20mM Phosphate, 150mM NaCl, buffered saline,
pH 7.0 with 0.05% Tween 20 from common laboratory chemicals.
Intended Use
A phosphate buffered saline solution, pH 7.0 can be used on the Dako Autostainer Plus.
Materials Required
1. Clean carboy, flask or beaker graduated to 1L
2. Deionized water
3. Magnetic stir plate with stir bar
4. Common laboratory chemicals
Chemical Manufacturer and Product No.
Sodium Phosphate, dibasic
(anhydrous) Sigma, S-0876
Sodium Phosphate, monobasic
(monohydrate) Fisher, S369-1
NaCl Sigma, S-9625
Tween 20 Dako, S1966
Sodium Hydroxide (NaOH), 50% w/w
solution Baker, 3727-03
Reagent Preparation
One Liter of Phosphate-Buffered Saline containing 0.05% Tween 20
1. Add approximately 500 mL of deionized water to a 1 L (1000 mL) carboy, flask or beaker.
2. Add 0.92 g of Sodium Phosphate, monobasic (monohydrate), 1.92 g of Sodium
Phosphate, dibasic and 8.77 g of NaCl and dissolve using a magnetic stir plate.
3. After all granules are dissolved adjust the solution to pH 7.0 with NaOH (50% w/w
solution). Allow the solution to continue to mix on the stir plate. Add the NaOH dropwise,
observing the pH constantly.