Dako Autostainer Plus - Manual (Page 6)

6 Dako Autostainer Plus | User Guide
The Options Screen .......................................................................................................... 36
Program Grid ................................................................................................................. 36
IHC Report..................................................................................................................... 37
Custom Options ............................................................................................................. 37
Section 8 | Entering Slide Information .............................................................................. 39
Slides Function.................................................................................................................. 39
Adding Slides................................................................................................................. 39
Deleting a Slide.............................................................................................................. 40
The SLIDE INFORMATION Button ................................................................................... 40
Entering Slide Information Through the SLIDE INFORMATION Button ........................ 41
Deleting Slide ID Information Through the SLIDE INFORMATION Button.................... 42
Deleting a Slide Identification ........................................................................................ 42
Deleting a Case # .......................................................................................................... 43
Deleting a Block ID ........................................................................................................ 43
Deleting a Specific Slide ................................................................................................ 44
Slide Rearrangement ........................................................................................................ 44
Rearranging slide positions on the Programming Grid .................................................. 44
Section 9 | Designing a Protocol ....................................................................................... 45
Protocol Elements ............................................................................................................. 46
Standard Protocol Elements include:............................................................................. 46
Rinse Buffer ................................................................................................................... 46
Rinse Water ................................................................................................................... 46
Standard Rinse Replacement........................................................................................ 46
Substrate-Batch ............................................................................................................. 47
Switch (*Swtch).............................................................................................................. 47
Creating and Editing a Protocol Template ..................................................................... 47
Saving a Protocol Template........................................................................................... 48
Deleting a Protocol Template ........................................................................................ 49
Using a New Protocol Template Without Saving It ........................................................ 49
Using a Saved Protocol Template ................................................................................. 49
Using a Saved Protocol Template for a Specific Auto Program .................................... 50
Selecting and Editing the Reagent Dispense Volume....................................................... 50
Selecting and Editing the Reagent Dispense Volume for All Steps in a Protocol.......... 50
Selecting and Editing the Reagent Dispense Volume for a Specific Step in a Protocol 51
Section 10 | Programming Slides ...................................................................................... 53
Defining Slide Specific Reagents ...................................................................................... 53
Defining Detection Reagents (Secondary, Tertiary, Substrate, etc.) ............................. 53
Defining Primary Antibodies .......................................................................................... 54
Assigning Positive and Negative Control Reagents ...................................................... 54
Negative Controls .......................................................................................................... 55
Assigning Negative Control Type to Antibodies............................................................. 55
Using the Negative Control Tool.................................................................................... 56
Defining Specific Rinses ................................................................................................ 56
Auto Programming ............................................................................................................ 57
Creating and Saving an Auto Program .......................................................................... 57
Deleting an Auto Programming Item.............................................................................. 58
Using an Auto Programming Item.................................................................................. 58
Getting Help with Auto Programming ............................................................................ 59
Copy/Paste........................................................................................................................ 59
Copy/Paste Individual Reagents.................................................................................... 59
Copy/Paste Entire Rows................................................................................................ 60
Editing a Protocol Step...................................................................................................... 60
Changing a reagent for a step: ...................................................................................... 60