Bio-Rad TC20™ Automated Cell Counter - Manual (Page 7)

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Table of Contents
Bio-Rad Laboratories Resources ............................................................................ii
Warranty .................................................................................................................ii
Safety Use Specifications and Compliance............................................................ iii
Chapter 1 — Introduction ................................................................................... 1
Instrument Overview ......................................................................................... 2
User Interface .................................................................................................... 3
TC20
™ Automated Cell Counter Specifications ................................................. 4
Kits, Reagents, and Accessories....................................................................... 4
Chapter 2 — Setting Up the TC20 Automated Cell Counter ............................ 7
System Components......................................................................................... 8
Unpacking the System Components................................................................. 8
Setting the Date and Time................................................................................. 8
Setting up Gating .............................................................................................. 9
Setting up Automatic Data Export and Sample Naming ................................. 10
Chapter 3 — Performing Cell Counts .............................................................. 11
Loading Slides ................................................................................................. 12
Preparing Samples .......................................................................................... 12
Preparing Samples without Trypan Blue Dye ............................................. 12
Preparing Samples with Trypan Blue Dye .................................................. 12
Counting Cells on the TC20 Automated Cell Counter ..................................... 13
Counting Samples with Multiple Cell Populations ........................................... 14
Sample Naming............................................................................................... 14
Current Count Options .................................................................................... 15
Using the Dilution Calculator ...................................................................... 15
Printing the Current Cell Count .................................................................. 16
Viewing the Image of Cells from the Current Count ................................... 16
Exporting the Image of Cells from the Current Count ................................ 16
Viewing the Histogram and Gating from the Current Count ....................... 17
Chapter 4 — Saved Count Results ................................................................... 19
Viewing Previous Counts ................................................................................. 20
Printing Previous Counts ................................................................................. 20
Exporting Previous Counts .............................................................................. 20
Automatic Image Export.................................................................................. 21
Deleting All Counts .......................................................................................... 22
Chapter 5 — Maintenance and Troubleshooting ............................................ 23
Performing the TC20 System Verification Test ................................................ 24
Cleaning the TC20 Automated Cell Counter.................................................... 25
Thermal Printer Maintenance ..................................................................... 25
Updating the Firmware .................................................................................... 26
Battery ............................................................................................................ 26
Troubleshooting ............................................................................................... 27
Ordering Information ......................................................................................... 28