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Bio-Rad TC20™ Automated Cell Counter User Manual

Page 23

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17

Performing Cell Counts

Fig. 16. Histogram for total cell count (sample
without trypan blue dye).

Fig. 17. Histogram for live and dead cell
count (sample with trypan blue dye).

To gate the histogram from the most recent count:

1. Use the down arrow key to select Histogram/gating from the Current count

screen, then press Enter.

2. When the histogram is displayed the low size gate is flashing and positions of gates

are displayed in microns in the lower right corner.

3. Use the up or down arrow keys to move the low gate to the desired position.

4. Press Enter to select the high gate. Use the up or down arrow key to move the high

gate to the desired position.

5. Press Enter to confirm position of both gates.

6. Gated cell count is displayed, press Enter to save it or press Home to start over.

Press Home anytime during the process to start over.

7. Press Enter to return to the Current count screen.

Note: If a new slide is inserted while the instrument is exporting an image, the instrument

queues the count until it completes the export. The instrument then detects the slide and

asks if it should start counting the sample in the new slide. If a new slide is inserted after

the image is exported, the instrument starts counting the sample in the new slide.

Viewing Histogram and Gating from the Current Count

The histogram of cell size distribution from the most recent count shows the

approximate cell diameters (in µm) on the X axis and the cell counts on the Y axis. The

cell counts are based on the 4 mm

2

imaged area. For samples without trypan blue, the

plot shows the total cell count (Figure 16). For samples with trypan blue, two plots are

used to show the number of live and dead cells (Figure 17).

Important: The TC20 cell counter accurately counts samples with single or mixed cell

populations. If you work with mixed cell population samples containing cells that vary

greatly in size you should enable the User defined gates in the Gating setup menu.

The histogram only shows approximate cell diameters; it is not intended to provide

precise cell diameters.