Bio-Rad TC20™ Automated Cell Counter - Manual (Page 11)

Fig. 3. Options screen.
4
Introduction
Options — for selecting additional operations
Options Screen
The Options screen (Figure 3) contains
the following choices:
Autosave/sample name — for setting
up the automatic export of cell images
to a USB flash drive and for setting up
sample naming preferences
System test — for verifying the
performance of the instrument;
a verification slide is needed for
this function
Set date/time — for setting the date
and time on the instrument, results are
associated with date/time stamps
Update firmware — for upgrading the firmware
Delete all counts — for deleting all counts stored in the instrument
TC20 Automated Cell Counter Specifications
Processing time: 30 sec (may increase for higher concentration cell suspensions)
Cell concentration range: 5 Ч 10 4 –1 Ч 10 7 cells/ml
Optimal concentration range: 1 x 10 5 –5 x 10 6 cells/ml
Cell diameter range: 6–50 µm
Dimensions (W x D x H): 19 Ч 15 Ч 25.4 cm
Weight: 2.2 kg
Kits, Reagents, and Accessories
TC20 kits, reagents, and accessories are specially designed for use with the
TC20 automated cell counter. These products are optimized to ensure accurate and
reproducible cell counts.
Counting Slides
The counting slide (Figure 4) is a disposable polygon plastic slide with a dual chamber
composed of polymethyl methacrylate (PMMA). Each slide is 75 Ч 25 Ч 1.8 mm (W x D x
H) with a chamber depth of 100 μm. Each enclosed chamber requires only 10 μl of
suspended cells. The volume counted in each chamber is 0.4 µl, equivalent to the
volume counted in four squares (each 1 × 1 mm) of a manual hemocytometer.
The slide can provide counts for two different samples or duplicates of the same sample,
one at a time. The chambers are labeled A and B, with an arrow next to each chamber
to indicate the direction of slide insertion. Both slide chambers can be loaded prior to
insertion in the TC20 cell counter for sequential counting.