Bio-Rad TC20™ Automated Cell Counter User Manual

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If automatic name serialization is enabled, you can select a name consisting of maximum

six characters, the last two fields are reserved for automatic numerical serialization.

Underscore symbol — create a space in the sample name

Dot (.) — indicates the end of the name, cursor will move to the Use name? field

To name a sample:

1. Insert a counting slide into the slide slot of the TC20 cell counter to initiate a cell count.

2. At the end of the count you will be

prompted to select a sample name

(Figure 14). Use the up or down

arrow key to scroll through the list of

characters until you find the correct

one. Then press Enter to confirm the

selection.

3. Cursor then moves on to the next

character field to be populated.

Repeat this until you have selected all

characters you need.

4. Cursor then moves onto the Use

name? field. Select Yes to use the

sample name, Rename to make

modifications, or No to not use the
sample name (the default CELLxxx. will be used instead). Then press Enter to confirm.

Current Count Options

Using the Dilution Calculator

The dilution calculator is used to calculate volume adjustments needed to

achieve the cell concentration required for the next experiment. If trypan blue was

used during the count, the instrument accounts for the 1:1 dilution with the dye in the

calculation results, and only the live cell concentration will be used for calculations.

To use the recent count as the starting cell concentration, use the following instructions:

1. From the Current count screen, use the down arrow key to select Dilution calculator.

2. Press Enter to continue.

3. Use the up or down arrow key to select a value, and press Enter to confirm the

selection. If the value of the desired cell count is higher than the starting cell count,

“Invalid dilution” is displayed on the screen.

4. Repeat step 3 until all the parameters have been set.

5. To recalculate the dilution, continue pressing Enter to go back to the field to be

modified. Subsequent changes to values automatically recalculate output results.

6. Once all the parameters have been set, the curser lands on the Print dilution

results field. To print the dilution results, use the down arrow key to select Yes and

then press Enter.

Performing Cell Counts

Fig. 14. Sample naming.