Bio-Rad Experion DNA Analysis Kits - Manual (Page 31)

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4.5.3 Conductivity Test
Perform this test, which uses the conductivity chip from the validation kit filled with water, to verify the
regulation of the current by the power supplies. Two different measurements are made (current and
voltage) for each electrode pin.
1. Under Tools > Diagnose Instrument, click Conductivity. The Instrument Diagnostics window
appears.
2. Follow the instructions in the Instructions field. Check the instruction after you complete the
command. When all instructions are checked, the Start button is enabled.
3. Click Start. A progress bar indicates the time remaining. Results appear in the Results field.
4.5.4 Focus Test
Perform this test, which requires the Experion validation kit, to verify the performance of the optics
and related electronics. It checks for correct operation of the limit switches, the existence of a good
fluorescence signal peak, laser %CV, background signal, and background noise.
1. Under Tools > Diagnose Instrument, click Focus. The Instrument Diagnostics window appears.
2. In the Step tab, follow the instructions in the Instructions field, checking them off as you proceed:
a. Record the horizontal offset (O/S) and signal level factor (INT, located on the back of the focusing
chip).
b. Enter those values under the Focus Chip Values tab.
3. When all instructions are checked, the Start button is enabled in the Step tab. Click Start.
A progress bar indicates the time remaining. Results appear in the Results field.
Experion Automated Electrophoresis System