Bio-Rad Experion DNA Analysis Kits - Manual (Page 21)

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Routine Cleaning Before RNA Analysis
Use this protocol, for example, at the start of a workday.
1. Fill one cleaning chip with 800 µl Experion electrode cleaner and another with 800 μl DEPC-treated
water. Gently tap the side of the chips to remove any trapped bubbles from the wells.
2. Place the cleaning chip with electrode cleaner on the chip platform, close the lid of the
electrophoresis station, and leave it closed for 2 min.
3. Replace the electrode cleaner chip with the chip containing the DEPC-treated water, close the lid,
and leave the chip in the electrophoresis station for 5 min to rinse the electrodes.
4. Replace the DEPC-treated water in the DEPC-treated water chip, and repeat the rinse step for 1 min.
5. Open the lid, remove the DEPC-treated water chip, allow the electrodes to dry for 1 min, and then
close the lid again.
6. Replace the water and electrode cleaner in the cleaning chips after each use to avoid contamination.
For storage, remove the water or electrode cleaner from each cleaning chip and store the chips in a
clean location.
Routine Cleaning After Each RNA Analysis
1. Fill the cleaning chip labeled DEPC with 800 µl DEPC-treated water. Gently tap the side of the
cleaning chip to remove any trapped bubbles from the wells.
2. Place the cleaning chip on the chip platform, close the lid of the electrophoresis station, and leave it
closed for 1 min.
3. Open the lid, remove the cleaning chip, allow the electrodes to dry for 1 min, and then close the lid.
4. Replace the water in the cleaning chip after use to avoid contamination. For storage, remove the
water from the cleaning chip and store the chip in a clean location.
3.1.3 Replacing the Electrode Manifold
If the electrode pins become damaged, contact Bio-Rad Technical Support to replace the entire
electrode manifold.
3.2 Priming Station
Periodically wipe the priming station clean using a soft, lint-free cloth and deionized water. Replace the
priming seal (catalog #700-7031) once a year or as needed (for example, if the seal becomes clogged
with gel, which may occur if the priming station is opened before priming is complete) to maintain
optimal performance. Display of the Check Seal message on the LCD indicates the priming seal may
need to be replaced.
To replace the priming seal (catalog #700-7031):
1. Wearing gloves to keep the seal clean, open the priming station, pull the priming seal tab forward
to remove it (Figure 3.4). Hold the tab as close as possible to the seal.
2. Position the new priming seal so the tab is located to the right, and insert the seal into the hole.
Use your thumb to push the seal back into position.
3. Reset the priming station by unplugging it for a few sec and then reconnecting the power cord.
The cylindrical portion of the seal has a ridge on one side that fits into a cutout in the opening of the
priming station. If the ridge and cutout are not lined up properly, the seal may be difficult to insert.
Experion Automated Electrophoresis System