Bio-Rad Experion DNA Analysis Kits - Manual (Page 18)

3.1 Electrophoresis Station
3.1.1 Cleaning the Electrophoresis Station
Clean the electrode manifold after every analysis (see Section 3.1.2, Cleaning the Electrodes, Method 2:
Routine Cleaning). For RNA analyses, perform the deep cleaning procedure on a regular basis
(for example, monthly) and whenever contamination is suspected.
Do not leave the cleaning chip in the electrophoresis station overnight.
There is no need to manually dry the electrodes after cleaning.
Never place objects other than chips on the chip platform beneath the electrode manifold.
Never place equipment in water or other liquids; ensure that no liquid is spilled onto or into the
electrophoresis station.
Keep the lid closed when the electrophoresis station is not in use.
Clean the outside of the electrophoresis station using a soft, lint-free cloth and deionized water.
To avoid electrical shock, never open the housing.
Refer servicing to qualified personnel only.
3.1.2 Cleaning the Electrodes
Two cleaning methods ensure the best results from the Experion electrophoresis station:
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Method 1 — deep-cleaning procedure; applies electrode cleaner directly to electrode pins
using lint-free foam swabs
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Method 2 — routine procedure; uses cleaning chips and is performed before or after
every run
Method 1: Deep Cleaning
This manual procedure applies Experion electrode cleaner directly to the 16 platinum pins in the
electrode manifold (Figure 3.1). Perform this procedure:
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Prior to first use of the Experion electrophoresis station
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Between a protein and RNA or DNA analysis, and between a DNA and RNA analysis
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Any other time RNase contamination is suspected or any contamination (for example, salt
deposits or other precipitates) is visible on the electrodes
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If a chip has been left in the electrophoresis station for an extended period of time
(for example, overnight)
Perform this procedure with the electrode manifold in place (installed in the electrophoresis station).
Depp cleaning requires the following supplies:
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Experion electrode cleaner (catalog #700-7252)
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Experion DEPC-treated water (catalog #700-7253)
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Lint-free cleaning swabs (catalog #700-7264)
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Keep the chip platform completely dry during this procedure. Cover the chip platform with plastic
wrap and a paper towel or foil to protect it from liquid (Figure 3.2).
Experion Automated Electrophoresis System