Bio-Rad C1000 Touch™ Thermal Cycler - Manual (Page 41)

C1000 Touch TM Thermal Cycler Manual
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The oligonucleotide sequence should be at least 14 bases long to give reasonable T
m
values.
Less than 14 bases uses the base pair counting method (Figure 26).
Figure 26. Breslauer interaction constants.
INTERACTION Delta (H) Delta (S) Delta (G)
AA TT 9.1 24 1.9
AT TA 8.6 23.9 1.5
AC TG 6.5 17.3 1.3
AG TC 7.8 20.8 1.6
TA AT 6 16.9 0.9
TT AA 9.1 24 1.9
TC AG 5.6 13.5 1.6
TG AC 5.8 12.9 1.9
CA GT 5.8 12.9 1.9
CT GA 7.8 20.8 1.6
CC GG 11 26.6 3.1
CG GC 11.9 27.8 3.6
GA CT 5.6 13.5 1.6
GT CA 6.5 17.3 1.3
GC CG 11.1 26.7 3.1
GG CC 11 26.6 3.1