Bio-Rad C1000 Touch™ Thermal Cycler - Manual (Page 14)

C1000 Touch TM Thermal Cycler Manual
5
Operating the Reaction Module Lid
The inner lid of the reaction module applies heat and force to the reaction vessel lids (caps or
tape) to produce consistent and successful reactions. Heating the inner lid prevents
condensation, while applying force seals the reaction to prevent evaporation.
WARNING! After a run, the heated inner lid can remain hot. Use caution when
opening and closing the lid.
To open the lid, use the following steps:
1. Turn the lid force knob counterclockwise to release the inner lid (Figure 6).
Figure 6. Turn the lid force knob counterclockwise to release the inner lid.
2. To open the lid, lift the lid lever up.
3. Lift the lid lever completely until the reaction module stays open without assistance.
To close the lid, follow these steps:
1. Push the lid lever down, making sure that the front of the lid is secured beneath the
housing.
2. Adjust the lid force by turning the lid force knob (Figure 7).
• Turn the knob clockwise to increase the lid force
• Turn the knob counterclockwise to decrease the lid force
3. Increase the lid force until the heated lid touches the reaction vessel. Continue to
increase the lid force further based on the following recommendations.
• If using tubes, increase the lid force by a quarter turn after the lid touches the
tubes
• If using plates, increase the lid force by a half turn after the lid touches the plate
NOTE: The position marks on the lid indicate quarter turns
WARNING! If the lid is tightened past the recommended force, tightening the lid
further will result in the knob slipping and causing a clicking sound. This is
designed as a warning that the lid force is past the recommended setting and may
result in damage to the reaction vessels. If this happens, decrease the lid force by
Lid force knob
(turn counterclockwise
to release the lid)