Bio-Rad C1000 Touch™ Thermal Cycler - Manual (Page 22)

C1000 Touch TM Thermal Cycler Manual
13
Protocol Parameters
Table 7 lists the parameters for temperature and gradient steps with the limits of the
parameters.
Table 7. Protocol steps and parameters of the C1000 Touch thermal cycler.
Step Name Parameters and Ranges Description
Temperature Temperature in °C: The target
temperature between 0.0 and
100.0°C in tenths of a degree Instructs the thermal cycler to ramp to
the target temperature and hold that
temperature for the specified amount
of time
Hold time: The hold time
between 1 sec and 18 hr in the
format of hr:min:sec. To enter an
infinite hold, touch the
∝ (infinite)
button
Gradient range Lower: The lower temperature
in the gradient. Enter a number
between 30.0 and 99.0°C in
tenths of a degree
Upper: The upper temperature
in the gradient. The maximum
temperature is 100°C. Enter a
temperature within 24.0°C of the
lower temperature
Instructs the thermal cycler to ramp to
the target temperature gradient across
the block and hold that temperature
gradient for the specified amount of
time
Time: The hold time between 1
sec and 18 hr in the format of
hr:min:sec. To enter an infinite
hold, touch the
∝ (infinite) button
Increment A temperature from –10.0 to
10.0°C per cycle in tenths of a
degree Applies only to a temperature step.
Instructs the thermal cycler to
increment (change) the target
temperature of a step with each cycle,
where a positive number increases the
temperature and a negative number
decreases the temperature
Ramp rate A number from 0.1 to 5°C per
sec Applies only to a temperature step.
Instructs the thermal cycler to ramp to
the target temperature at the specified
ramp rate in that step
Extend A time from –60 to 60 sec per
cycle Applies to both temperature and
gradient steps. Instructs the thermal
cycler to extend the hold time with
each cycle. A positive number
increases the hold time and a negative
number decreases the hold time
Beep (No parameters) Applies only to a temperature step.
Instructs the thermal cycler to beep to
signal that the thermal cycler has
reached the target temperature for that
step