Bio-Rad C1000 Touch™ Thermal Cycler - Manual (Page 36)

C1000 Touch TM Thermal Cycler Manual
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4 Protocol AutoWriter
Read this chapter for information on using the Protocol AutoWriter.
• Creating a protocol with the Protocol AutoWriter (page 27)
• Overview of the Protocol AutoWriter (page 29)
• The T
a
calculator (page 30)
Creating a Protocol with the Protocol AutoWriter
Follow these steps to use the Protocol AutoWriter for creating a new protocol:
1. Touch the Protocol AutoWriter button on the home screen.
2. Select the enzyme to be used for the reaction.
The default selection for the polymerase is iTaq TM DNA polymerase. If iProof TM DNA
polymerase is used, touch the iProof radio button to select iProof polymerase.
NOTE: If iProof polymerase is selected, the protocol automatically adds 3°C to the
T
a
value. If the Other radio button is selected, additional options are presented,
such as Hot Start Activation (Figure 24).
Figure 24. If Other is selected for the enzyme, additional options are presented.
3. Touch the Amplicon Length button and enter the amplicon length in base pairs (bp).