Metrohm 881 Compact IC pro – Anion - Manual (Page 98)

5.12 Separation column
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881 Compact IC pro – Anion
6 Inserting the new connecting piece
￭ Insert the connecting piece (39-2) into the housing (39-4) in such
a way that the connector 1 is on top and the three pins of the
connecting piece fit into the corresponding recesses on the hous-
ing.
￭ Reattach the union nut (39-1) onto the coupling and tighten by
hand..
7 Connecting and conditioning the suppressor
￭ Reconnect all capillaries of the suppressor to the IC system.
￭ Before switching the suppressor over for the first time, rinse the
three suppressor units with solution for 5 minutes.
5.12 Separation column
5.12.1 Separating efficiency
Which analysis quality can be attained, depends to a great extent on the
separating efficiency of the separation column used. The separating effi-
ciency of the selected separation column must be sufficient for the analy-
sis problems present. If difficulties occur, you should always first check the
quality of the separation column by recording a standard chromatogram.
You can find detailed information on the separation columns available
from Metrohm in the leaflet provided along with your separation column,
in the Metrohm IC-Column Program (available via your Metrohm
agent) or in the Internet at
http://www.metrohm.com in the product area
Ion chromatography. You can request free information on special IC appli-
cations in the corresponding "Application Bulletins" or "Application
Notes", which are available in the Internet at
http://www.metrohm.com
in the Applications area or via the Metrohm agent responsible.
5.12.2 Protection
To protect the separation column against foreign particles, which can
affect the separating efficiency, we recommend that both the eluent and
the samples undergo a microfiltration (filter 0.45 µm) before being aspira-
ted via the aspiration filter (6.2821.090).
We recommend always to use a guard column (see Chapter 3.18, page
54). This protects the separation column and considerably increases its
service life. Information regarding which guard column is suitable for your
separation column can be found in the Metrohm IC Column Program
(which is available from your Metrohm agent), the leaflet provided along
with your separation column, the product information on the separation