Leica Biosystems RM2125 RTS - Manual (Page 26)

26 Instructions for Use, V2.1 – 10/2012
5.7 Sectioning
Always rotate the handwheel at a uniform speed. The rotation speed of the handwheel must be
adapted to suit the hardness of the specimen.
For harder specimens, use a slower speed.
CAUTION: The handwheel continues turning if it has been rotated very quickly and then
released – this can cause crushing or other injuries!
5.8 Changing specimens
Lock the handwheel and cover the knife edge with the knife guard prior to any manipulation of
knife or specimen, as well as prior to changing specimens and during all work breaks!
• Move the specimen to the upper end position by turning the hand-
wheel and engage the handwheel lock.
• Cover the sectioning edge with the knife guard.
• Remove the specimen from the specimen clamp and mount a new
specimen.
• Run the object clamp with the coarse feed back far enough until the
new specimen can start being cut.
• To do so, move the knife holder laterally on the knife holder base ac-
cordingly (see Chap. 5.9.7 ) or, when using the knife holder base with-
out lateral movement, move the knife or disposable blade in the knife
holder.
• For sectioning, turn the handwheel (15) evenly in a clockwise direction.
• Pick up the sections and mount them on microscope slides.
5. Operation
• Set the desired section thickness using the
section thickness setting knob (33) at the
front of the microtome on the right, or check
the set value in the viewing window (34).
The red indicator (38) indicates the selected
section thickness (on the scale).
• Use a different area of the cutting edge for
trimming than for sectioning.
Fig. 18
34
33 38