Leica Biosystems RM2125 RTS - Manual (Page 21)

21 Leica RM2125 RTS
5.4 Specimen retraction (specimen orientation)
5. Operation
Fig. 13
To prevent the knife or blade from touching the overlapped specimen while returning to the
upper end position, the specimen is retracted 40 µm when retraction is activated.
The user can switch the specimen retraction ON or OFF.
To do so, use an Allen key with handle, No. 4 (included in the delivery package) and turn the
screw depicted in the detail picture (Fig. 13a) so that the red dot indicates "OFF" = retraction is
disabled. Red dot on "ON" means = retraction is switched on.
Important if retraction is enabled:
The specimen must NOT be oriented or approached in the retraction phase (if the black
dot on the handwheel is visible – see enlarged detail Fig. 13 – you are in the sectioning
phase)! The previously retracted specimen will advance by the retraction value PLUS
the selected section thickness before the next section.
There is a danger of the specimen and knife being damaged by a section that is too
thick.
The same applies to the "rocking mode", during which the specimen is trimmed by
rocking motion (no complete rotation of the handwheel).
Carry out "rocking mode" ONLY in the sectioning phase – NEVER in the retraction
phase!
Fig. 13a
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