C.B.S. Scientific HTLE-7002 - Manual (Page 24)

C. B. S. œ Scientific 24 HTLE-7002
29. The plate should be oriented so that the
dye and sample marks are on a vertical
axis from top to bottom, which should be
perpendicular to the electric field (edges
of the wicks). Remember that the anode
(+red) will be on the left, and the
cathode (-black) on the right. Most of
the peptides will migrate toward the
cathode or to the right during
electrophoresis if using either pH1.9 or
4.72 buffers. (PAAs go left). Next,
place the wicks so they overlap onto the
cellulose by about 1cm. Cover the plate
and wicks with the top polyethylene
sheet, followed by the Teflon pad, then
the neoprene pad (Fig. X).
30. Close the lid and again secure the pins.
Next, turn the air pressure up to 10psi
as before and make sure that the water
supply is on for cooling the device
during the run. (See Fig. Y).
31. Close the safety cover of the apparatus,
then set the voltage timer and turn
mains switch and the power supply on:
For this run, with pH1.9 buffer, we ran
for 25min at 1.0kV (
∼21mA (Fig. Z).
After the run is complete, turn the
power supply off and the mains power,
then release the air pressure.
Disassemble the apparatus and
carefully remove each layer then the
plate.