C.B.S. Scientific HTLE-7002 - Manual (Page 2)

C. B. S. œ Scientific 2 HTLE-7002
TABLE OF CONTENTS
Page
Important User Information 3-4
Section 1
General Information
1.1 Introduction 5
1.2 Specifications 6
1.3 Overall Sizes and Shipping Weight 6
1.4 Safety 6
Section 2 Description of parts
2.1 Unpacking 7
Section 3 Instructions for Use
3.1 HTLE-7002 Unit Preparation 8-9
3.2 Preparation of Samples 10-13
Elution from Polyacrylamide Gels 10-11
Precipitation 11-12
Proteolytic Digestion and Oxidation 12
Washing and Lyophilization 13
3.3 First Dimension: Thin Layer Electrophoresis 13-24
Choice & Preparation
of
Electrophoresis Buffers 14
Preparation of Thin Layer Plates 15-16
Sample & Marker Dye Application 16-18
Preparing the Apparatus
&
Electrophoresis 18-25
3.4 Second Dimension: Thin Layer
Chromatography 26
Choice & Preparation of
Chromatography
Buffers 26
Preparing the Chromatography
Tank
& Chromatography 27
3.5 Phosphoamino Acid Analysis 27-28
3.6 Autoradiography & Fluorography 29
3.7 References 29
Section 4 Maintenance of Equipment
4.1 Care and Handling 30
4.2 Maintenance 30
Section 5 Ordering Information 31
Section 6 Appendix 32-42
Illustrations & Figures
a. Diagram of Electrophoresis components
b. Stencils
1. PAA:2-D PAA
2. Stencil for 2-D PAA
3. Stencil Outline for TLC plate
c. Flow Switch, fluid operation
d. Air pressure electric interlock
Rev 8-15-03