C.B.S. Scientific HTLE-7002 - Manual (Page 15)

C. B. S. œ Scientific 15 HTLE-7002
B. Initial Preparation of Thin Layer Cellulose Plates
1.
Select a thin layer cellulose plate. We
use 100
μm x 20x 20cm cellulose plates
(E.M. Laboratories) (Fig. A). To do this
first make sure that the cellulose layer is
of a uniform thickness with an even
surface that does not have any gouges
or nicks.
2. Next, place the plate on a light box and
illuminate from beneath to see if the
thickness of cellulose is even across the
area of electrophoresis and
chromatography (Fig. B). Based on this
criteria most plates are suitable,
although we often come across boxes
(25 plates) that contain several plates
with an unusable cellulose surface. We
also recommend using one orientation
for all the plates in the box.
3. Use the stencil outline in the Appendix
(Section 6) to make several orientation
marks on the plate. Place the outline on
a light box (Fig. C).