C.B.S. Scientific HTLE-7002 - Manual (Page 22)

C. B. S. œ Scientific 22 HTLE-7002
E. Final Preparation of TLC Plate
23. The plate needs to be wetted with the
same buffer used in the tanks before it is
ready for electrophoresis. We use a
special blotter that wets the plate while
also concentrating the sample. It is
composed of two sheets of Whatman
3MM paper sewn together and has two
holes about 1.5-2.0cm in diameter cut at
positions corresponding to the sample
and dye application marks (use the
template in the appendix to align the
holes). Soak the blotter in buffer in a
large tray (Fig. S).
24. Let the blotter drain to remove excess
liquid (Fig. T) then blot it against dry
paper until it is only slightly damp using
gloved hands.
25. Blotters can be reused until they literally
fall apart. Used blotters should be
labeled depending on which buffer they
have been used for, and rewet only with
the same buffer. Place the TLC plate on
a flat surface, cellulose side up, then
carefully place the damp blotter down on
the plate (Fig. U).