Luminex MAGPIX User Quick Guide - Manual (Page 25)

Problem Resolution
VER (strip well B)
Verify that reagent is freshly vortexed. Use 6 drops of reagent.
(1x) Rinse (reservoir D)
Fluidics 1 (strip well C)
Verify that reagent is freshly vortexed. Use 6 drops of reagent.
Fluidics 2 (strip well D)
Verify that reagent is freshly vortexed. Use 6 drops of reagent.
(3x) Rinse (reservoir D)
Check the Cal/Ver icon in the System Monitor Bar located at the bottom of the
xPONENT screen to confirm that the operation has been completed
successfully.
3. If performance verification continues to fail, contact Luminex Technical
Support.
First well has a low
bead count 1.On the xPONENT 4.2 Admin page, verify that Run Rinse Before Batch
Starts is checked.
2. Rerun the plate.
3. Close and restart xPONENT.
4. Change the sample load volume (see procedure below).
Error at xPONENT
start If you configured your PC to auto-start xPONENT when booting, disable this
function.
Probe Positioning
1. Cover a 96-well plate with foil and insert it into MAGPIX.
2. Access the Probe and Heater tab of the Maintenance page.
3. Select a well and click Probe Down.
4. Move the probe up and eject the plate.
5. Verify that the probe pierced the well you selected at its center.
Changing Sample Load Volume
NOTE: You can change the sample load volume only after pausing the
batch. You can pause it manually while a procedure is running, or
you can select Single Step on the Batches page to create an
automatic pause after each well.
1. While the batch is paused, click Chg Vol on the Current Batch tab.
2. Enter the new volume (in µL) and click OK.
MAGPIX ® User Quick Guide 4.2
20