Luminex MAGPIX User Quick Guide - Manual (Page 24)

Problem Resolution
(1x) Clean with 0.1 N NaOH (reservoir C)
(3x) Rinse (reservoir D)
Run Calibration routine. Verify that reagent is freshly vortexed. Use 6 drops of
reagent.
4. If calibration continues to fail, contact Luminex Technical Support.
Performance
Verification Failure 1. Sonicate and flush the sample probe using distilled water. Observe the probe
calibration for positional accuracy (see procedure below). Verify all fittings are
secured in place (see flowchart below).
Refer to the MAGPIX Calibration and Performance Verification Troubleshooting
Guide flowchart below for detailed information about the fittings.
2. If system continues to fail, create and run the enhanced start-up routine
outlined below.
Create routines in the xPONENT Maintenance page Cmds & Routines tab.
Approximate run time: Thirty minutes
Sonicate and flush the sample probe (flush from narrow to wide end) using
distilled water.
(4x) Rinse (reservoir D)
(3x) Alcohol Flush with 70% isopropanol or ethanol alcohol (reservoir B)
(1x) Alcohol Flush with DI water (reservoir A)
(1x) Sanitize with 0.1N NaOH (reservoir C)
(2x) Clean with 0.1N NaOH (reservoir C)
(2x) Rinse (reservoir D)
(1x) Alcohol Flush with70% isopropanol or ethanol alcohol (reservoir B)
(1x) Prime
(4x) Rinse (reservoir D)
CAL (strip well A)
Verify that reagent is freshly vortexed. Use 6 drops of reagent.
(3x) Rinse (reservoir D)
Troubleshooting
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