BUCHI Pump Module C-605 - Manual (Page 32)

6 Operation
32 Pump Combinations Operation Manual, Version E
Anfangskonzentration
Endkonzentration
Dauer
Segment 1 Segment 2 Segment 3
Konzentration B (%)
Zeit t (min)
Concentration B(%)
Segment 1 Segment 2 Segment 3
Start concentration
End concentration
Time t (min) Duration
Fig. 6.5: Systematical gradient
The program number under which the program is to be saved can be selected at will between P 01
and P 10. This option allows an existing program to be loaded, edited and/or viewed and saved under
a new number, whereby the original program remains unchanged.
Creating a new program, example:
Prerequisites:
Two pump modules, e.g. C-605, are installed, each with a solvent. No solvent Valve Set is installed.
A gradient program P02 is created that initially runs for 25 minutes isocratically with pure Eluent A,
then, for 30 minutes, the concentration of Eluent B is continuously increased to 35% and finally this
concentration B is maintained for 20 minutes. Eluent A could e.g. be hexane, and Eluent B ethyl
acetate.
The pressure is 50 bar and the flowrate is 10 ml/min.
25 55 75
100
0
Segment 1 Segment 2 Segment 3
Zeit t (min)
Konzentration B (%) Concentration B(%)
Segment 1 Segment 2 Segment 3
100
Time t (min)
0
25 55 75
Fig. 6.6: Systematical gradient, example
To create a new program, the following settings are necessary:
• Pump B is selected: Pump B
Yes
• No valve is connected : Valve No