Bio-Rad Model 550, Ultramark, and Benchmark Microplate Reader Accessories User Manual

Page 37

8. : “RPLATE” - read plate; arguments , ,

: mix - Mixing time before reading (in seconds) ; ASCII encoded

decimal (0-9)
wp1 - Measurement filter position; ASCII encoded decimal (1-4)

wp2 - Reference filter position; ASCII encoded decimal (1-4)
Optional; only present for Dual wavelength reading

: “ERE”

9. : “RTPLATE” - retransmit the last plate read; no arguments

: “ERE”

Absorbance Data

< Mes.data> or

Ref. data present for dual wavelength readings.

“BIO-RAD MODEL 550 READER”

: “Mes. filter:”

“Ref. filter:” - Present for Dual wavelength reading

: “ . begin” <8 rows of 12 wells of abs. values>

“.end”
Measurement wavelength absorbance values are in ASCII encoded
floating point format. Each absorbance value is separated by a space.
Row1=wells (A1-A12), Row2=wells (B1-B12), .... Row8=wells (H1-
H12).

: “ . begin” <8 rows of 12 wells of abs. values>

“.end”
Reference wavelength absorbance values are in ASCII encoded
floating point format. Each absorbance value is seperated by a space.
Row1=wells (A1-A12), ROW2=wells (B1-B12, ....Row8=wells (H1-
H12)

: The checksum is a two byte sum in ASCII encoded decimal (0-255)

of all the ASCII characters transmitted in the measurement or
reference data block. The checksum does not include the character
string “.begin” in the checksum calculations.

The following is an example of both and :

“. begin”
“ 0.101 0.102 0.103 0.104 0.105 0.106 0.107 0.108 0.109 0.110 0.111 0.112”
“ 0.201 0.202 0.203 0.204 0.205 0.206 0.207 0.208 0.209 0.210 0.211 0.212”
“ 0.301 0.302 0.303 0.304 0.305 0.306 0.307 0.308 0.309 0.310 0.311 0.312”
“ 0.401 0.402 0.403 0.404 0.405 0.406 0.407 0.408 0.409 0.410 0.411 0.412”
“ 0.501 0.502 0.503 0.504 0.505 0.506 0.507 0.508 0.509 0.510 0.511 0.512”
“ 0.601 0.602 0.603 0.604 0.605 0.606 0.607 0.608 0.609 0.610 0.611 0.612”
“ 0.701 0.702 0.703 0.704 0.705 0.706 0.707 0.708 0.709 0.710 0.711 0.712”
“ 0.801 0.802 0.803 0.804 0.805 0.806 0.807 0.808 0.809 0.810 0.811 0.812”

“ . end”

Note: Absorbance values greater then 3.000 shall be transmitted as an asterisk.