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Recommended materials – Bio-Rad Human Metabolic and Hormone Assays User Manual

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Item

Bio-Plex

®

200 system or Luminex system with HTF

Bio-Plex validation kit

Note: Run the validation kit monthly to ensure optimal

performance of fluidics and optics systems

Bio-Plex calibration kit

Note: Run the calibration kit daily to standardize

fluorescence signal

Bio-Plex Pro wash station

For use with magnetic bead–based assays only

Bio-Plex Pro II wash station

For use with both polystyrene (nonmagnetic) and magnetic

bead–based assays

Bio-Plex handheld magnetic washer

For use with magnetic bead–based assays only

Bio-Plex Pro flat bottom plates (40 x 96-well)

For magnetic separation on the Bio-Plex Pro wash station

Titertube

®

micro test tubes

For preparing replicate standards, samples, and controls

prior to loading the plate

Microtiter plate shaker

IKA MTS 2/4 shaker for 2 or 4 microplates

or

Barnstead/Lab-Line Model 4625 plate

shaker (or equivalent capable of 300–1,100 rpm)

Aurum

vacuum manifold

For vacuum filtration

BR-2000 vortexer

Reagent reservoirs, 25 ml

For capture beads and detection antibodies

Reagent reservoir, 50 ml (for reagents and buffers)

Pall Life Science Acrodisc: 25 mm PF syringe filter

(0.8/0.2 µm Supor membrane)

Filter plate, 1 x 96 with clear plastic lid and tray

Ordering Information

Bio-Rad catalog #171-000205

Bio-Rad catalog #171-203001

Bio-Rad catalog #171-203060

Bio-Rad catalog #300-34376

Bio-Rad catalog #300-34377

Bio-Rad catalog #170-20100

Bio-Rad catalog #171-025001

Bio-Rad catalog #223-9390

IKA catalog #320-8000

VWR catalog #57019-600

Bio-Rad catalog #732-6470

Bio-Rad catalog #166-0610

VistaLab catalog #3054-1002

or

VistaLab catalog #3054-1004

VistaLab catalog #3054-1006

Pall Life Sciences

catalog #4187

Bio-Rad catalog #171-304502

Other: 15 ml polypropylene tubes for reagent dilutions, calibrated pipets, pipet tips, sterile
distilled water, aluminum foil, absorbent paper towels, 1.5 or 2 ml microcentrifuge tubes, and
standard flat bottom microplate (for calibrating vacuum manifold).

Table 2. Recommended materials.