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Bio-Rad Human Metabolic and Hormone Assays User Manual

Page 30

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28

Possible Causes

Low Bead Count

Vacuum on for too long when
aspirating buffer from wells

Reader is clogged

Low Signal or Poor Sensitivity

Standards reconstituted
incorrectly

Detection antibody or
streptavidin-PE prepared incorrectly

High Background Signal

Incorrect buffer was used
(for example, assay buffer used
to dilute standards)

Accidentally spiked blank wells


Detection antibodies or
streptavidin-PE incubated too long

Possible Solutions

Do not apply vacuum to the filter
plate for longer than 10 sec after the
buffer is completely drained from
each well.

Refer to the troubleshooting guide
in the Bio-Plex system hardware
instruction manual (bulletin
#10005042).

Follow the standard preparation
instructions carefully.

Check your calculations and be
careful to add the correct volumes.

Use standard diluent to dilute
standards.

Do not add any antigens to the
blank wells.

Follow the procedure incubation
time precisely.