Bio-Rad Human Metabolic and Hormone Assays - Manual (Page 26)

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2. Click Run Protocol — on the pop-up screen, select Load Plate and
click OK to start acquiring data.
3. Use the Wash Between Plates command after every plate run
to reduce the possibility of clogging the instrument.
4. If acquiring data from more than one plate, empty the waste bottle and
refill the sheath bottle after each plate (if HTF are not present). Select
Wash Between Plates and follow the instructions. Then repeat the
Prepare Protocol and Acquire Data instructions.
5. When data acquisition is complete, select Shut Down and follow
the instructions.
Data Analysis
Quality Controls
If the quality controls were run in the assay plate, open the results (.rbx)
file, click Report Table, and locate the control wells. Compare the
observed concentrations against the lot-specific control ranges in the
product data sheet.
Note: Expected control ranges are provided for reference and should be
used as general guidelines. Actual results may vary for some operators.
If the controls do not fall within the expected ranges, please refer to the
troubleshooting section for possible causes and solutions.
Removing Outliers
Outliers are identified as standard data points that do not meet accuracy
or precision requirements and should be considered invalid when
performing curve fitting. As such, they should be removed to generate
a more realistic and accurate standard curve. This may result in an
extended assay working range and allow quantitation of samples that
might otherwise be considered out of range (OOR).
In Bio-Plex Manager software version 6.0 and higher, outliers can be
automatically removed by selecting the Optimize button in the Standard
Curve window. In earlier versions of the software, outliers can also be
manually selected in the Report Table. Visit online Help to learn more about
the standard curve optimizer feature and how outliers are determined.