Bio-Rad Human Metabolic and Hormone Assays - Manual (Page 24)

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3. Click Format Plate and format the plate according to the plate layout
template (located on page 31) created for the assay. To modify the
plate layout, follow the steps below (see Figure 4).
a. Select the Plate Formatting tab.
b. Select the standards icon
S
and drag the cursor over all the
wells that contain standards. Repeat this process for blanks
B
,
controls
C
, and samples
X
.
4. Click Enter Standards Info in the Protocol Settings bar.
a. Enter the highest concentration of each analyte in the top row
(labeled S1) of the table. S1 concentration information is listed in the
product data sheet.
b. Enter a dilution factor of 3 and click Calculate. The concentrations for
each standard point will be populated for all analytes in the table.
c. Optional: enter the lot number of the vial of standards into the
Standard Lot box and click Save.
Fig. 4. Plate formatting.