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Kit contents and storage, Storage and stability, The bio-plex pro – Bio-Rad Human Metabolic and Hormone Assays User Manual

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Kit Contents and Storage

The Bio-Plex Pro

RBM IGF and IGFBP assays are available in

a convenient kit format that includes assay, reagent, and diluent
components in a single box (Table 1). All other recommended materials
are listed in Table 2.

Table 1. Contents of 1 x 96-well kits.

Storage and Stability

Kit contents should be stored at 2–8°C and never frozen. Coupled
magnetic capture beads and streptavidin-PE should be stored in the
dark. All components are guaranteed for a minimum of six months from
the date of purchase when stored as specified.

Volume after

Component

Quantity

Volume

Reconstitution or Dilution

Capture beads (1x)

1 tube

1.4 ml

Detection antibodies

1 vial

Lyophilized

4.8 ml

Standards mix

1 vial

Lyophilized

150 µl

Control 1 (high)

1 vial

Lyophilized

100 μl

Control 2 (low)

1 vial

Lyophilized

100 μl

Blocking buffer

1 vial

Lyophilized

1.5 ml

Standard diluent

1 vial

Lyophilized

1.0 ml

Sample dilution buffer 2

1 bottle

100 ml

Assay buffer (10x)

1 bottle

60 ml

600 ml

Streptavidin-PE (10x)

1 tube

250 μl

2.5 ml

Assay plate (96-well flat bottom)

1 plate

Plate seals

1 pack of 3

Assay quick guide

1 sheet

Product data sheet

1 sheet