Bio-Rad Human Metabolic and Hormone Assays - Manual (Page 10)

8
lmportant Considerations
Instruments and Software
The assays described in this manual are compatible with all currently
available Luminex-based life science research instruments. Assays can
be read and analyzed with either Bio-Plex Manager
™ software or Luminex
xPONENT software.
Assay Procedures
Pay close attention to vortexing, shaking, and incubation times and to
Bio-Plex
® reader PMT (RP1) setting, as these have been optimized specifically
for each assay panel.
Assay Quick Guide
Each assay kit comes complete with a printed Bio-Plex Pro RBM IGF
and IGFBP Assays Quick Guide (bulletin #10042014), which can be
used to set up and prepare a full 1 x 96-well assay plate. Users can also
download a copy at www.bio-rad.com/bio-plex.
Bead Regions and Multiplexing Compatibility
n
Bead regions for all analytes are listed in the Read Plate section
n
Do not mix analytes between different panels, or with other Bio-Plex
assay panels or reagents
Detailed Instructions
The following pages provide detailed instructions for each step of the
assay procedure, including preparation, running the assay, and reading the
plate with Bio-Plex Manager
™ and Luminex xPONENT software.