Bio-Rad Human Metabolic and Hormone Assays - Manual (Page 12)

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2. Prepare Instrument
These directions are specific for the Bio-Plex ® 100/200 reader. To prepare
either a Bio-Plex 3D or Bio-Plex ® MAGPIX ™ reader, consult their respective
user manuals.
Start up and calibrate the Bio-Plex system with Bio-Plex Manager ™
software prior to setting up the assay. The calibration kit should be run
daily or before each use of the instrument to standardize the fluorescent
signal. For instructions on using other xMAP system software packages,
contact Bio-Rad Technical Support.
Note: While the instrument is warming up, bring the 10x assay buffer and
sample dilution buffer to room temperature. Keep other items on ice until
needed. Also, begin to thaw frozen samples.
The validation kit should be run monthly to ensure performance of fluidics
and optics systems. Refer to either the software manual or online Help for
directions on how to conduct validation.
Start Up System (Bio-Plex 100, 200, or Similar)
1. Empty the waste bottle and fill the sheath fluid bottle before starting
if high throughput fluidics (HTF) are not present. This will prevent fluidic
system backup and potential data loss.
2. Turn on the reader, XY platform, and HTF (if included). Allow the
system to warm up for 30 min (if not already done).
3. Select Start up and follow the instructions. If the system is idle
for 4 hr without acquiring data, the lasers will automatically turn off.
To reset the 4-hr countdown, select Warm up
and wait for the
lasers/optics to reach operational temperature.
Calibrate System
1. Select Calibrate and confirm that the default values for CAL1
and CAL2 are the same as the values printed on the bottle of Bio-Plex
calibration beads. Use the Bio-Plex system low RP1 target value.