Bio-Rad Human Metabolic and Hormone Assays - Manual (Page 23)

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To create a new protocol, select File, then New from the main menu.
Locate and follow the steps under Protocol Settings.
1. Click Describe Protocol and enter information about the assay (optional).
2. Click Select Analytes and create a new panel.
a. Click Add Panel in the Select Analytes toolbar. Enter a new
panel name. Select Bio-Plex Pro Assay Magnetic from the assay
dropdown menu. If using Bio-Plex Manager version 5.0 or lower, select
MagPlex from the assay dropdown menu.
b. Click Add. Enter the bead region number and name for the first
analyte. Click Add Continue to repeat for each analyte in the assay.
Refer to the bead regions in parentheses ( ) listed on the peel-off label
provided with the standards.
For reference, bead regions for the individual assays are listed in
Table 10.
c. Click Add when the last analyte has been added and click OK to save
the new panel.
d. Highlight analytes from the Available list (left) and move to the
Selected list (right) using the Add button. To move all analytes at
once, simply click Add All.
e. If some of the analytes need to be removed from the Selected
list, highlight them and select Remove. If desired, it is possible to
rename the panel by clicking on Rename Panel and entering a
new panel name.
IGF Panel Bead Region IGF BP Panel Bead Region
IGF-1 21 IGFBP-1 48
IGF-2 54 IGFBP-2 65
IGFBP-3 33
IGFBP-4 76
IGFBP-5 51
IGFBP-6 78
IGFBP-7 37
Table 10. Bead regions for Bio-Plex Pro RBM IGF and IGFBP assays.