Bio-Rad Bio-Plex Pro™ Rat Cytokine, Chemokine, and Growth Factor Assays - Manual (Page 9)

7
Human, mouse, and rat diabetes
0 2 4 6 8 10
Mouse cytokine (group III) analytes (20x)
Human and mouse cytokine (groups I, II)
analytes (10x)
10 9 8 7 6 5
Important Considerations
Instruments and Software
The cytokine assays described in this manual are compatible with all
currently available Luminex-based life science research instruments.
Assays can be read and analyzed with either Bio-Plex Manager
™ software
or Luminex xPonent software.
Assay Procedures
Pay close attention to vortexing, shaking, and incubation times and to
Bio-Plex
® reader PMT (RP1) setting, as these have been optimized
specifically for each assay panel.
Assay Quick Guide
Each assay kit includes a printed Bio-Plex Pro ™ Assay Quick Guide (bulletin
#10024985), which can be used to prepare and run a full 1 x 96-well assay
plate. Users can also download a copy at www.bio-rad.com/bio-plex.
Bead Regions
Bead regions for all analytes are listed in the Read Plate section.
Multiplexing Compatibility
For human and mouse, the maximum number of singleplex diabetes
and cytokine analytes that may be mixed is limited by the 10x cytokine
antibody stock concentrations as shown in the table below.
Table 3. Maximum number of singleplex cytokine and diabetes analytes that may
be multiplexed.