Bio-Rad Bio-Plex Pro™ Rat Cytokine, Chemokine, and Growth Factor Assays - Manual (Page 3)

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Introduction
Cytokines, chemokines, and growth factors are a diverse group of cell
signaling proteins expressed and secreted by virtually all cell types,
including cells of endothelial, epithelial, and immune origin. These proteins
interact with specific receptors on target cells to mediate important
physiological responses such as growth, immunity, inflammation,
and hematopoiesis. Dysregulation of expression is associated with
pathological conditions ranging from cancer and diabetes to infection and
autoimmune disease.
Bio-Plex Pro ™ assays enable researchers to quantify multiple protein
biomarkers in a single well of a 96-well plate in 3–4 hours. These robust
immunoassays require as little as 12.5 μl serum or plasma or 50 μl cell
culture supernatant or other biological fluid. The use of magnetic (MagPlex)
beads allows researchers to automate wash steps on a Bio-Plex Pro (or
similar) wash station. Magnetic separation offers greater convenience and
reproducibility compared to vacuum filtration.
For more information please visit www.bio-rad.com/bio-plex.
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