Bio-Rad Bio-Plex Pro™ Rat Cytokine, Chemokine, and Growth Factor Assays - Manual (Page 23)

21
Preparing 1x coupled beads from 20x stock (includes 20% excess volume)
Table 12. Premixed panel or one singleplex assay.
Table 13. Mixing two singleplex assays or a premixed panel + singleplex assay.
Table 14. Preparing 1x beads from two stocks at different concentrations. Mixing human
insulin (20x) with human IL-6 (10x) is one example*.
* Due to differences in dilution factors, it is not possible to multiplex adiponectin, adipsin,
VCAM-1, or ICAM-1 with other diabetes or cytokine assays.
# of Wells 20x Beads, µl Assay Buffer, µl Total Volume, µl
96 288 5,472 5,760
48 144 2,736 2,880
20x Beads, µl 20x Beads, µl
# of Wells Singleplex #1 Singleplex #2 Assay Buffer, µl Total Volume, µl
96 288 288 5,184 5,760
48 144 144 2,592 2,880
20x Beads, µl 10x Beads, µl Assay Total
# of Wells Diabetes Cytokines Buffer, µl Volume, µl
96 288 576 4,896 5,760
48 144 288 2,448 2,880