Bio-Rad Bio-Plex Pro™ Rat Cytokine, Chemokine, and Growth Factor Assays - Manual (Page 30)

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8. Read Plate
Bio-Plex Manager ™ software is recommended for all Bio-Plex Pro ™ assay
data acquisition and analysis. Instructions for Luminex xPONENT software
are also included. For instructions using other xMAP system software
packages, contact Bio-Rad Technical Support or your regional Bio-Rad
field applications specialist.
Prepare Protocol in Bio-Plex Manager
Software v 6.0 and Higher
The protocol should be prepared in advance so that the plate is read as
soon as the experiment is complete.
A protocol file specifies the analytes used in the reading, the plate wells
to be read, sample information, the values of standards and controls, and
instrument settings.
Bio-Plex Manager software contains protocols for most Bio-Plex ® assays.
Choose from available protocols or create a new protocol. To create a new
protocol, select File, then New from the main menu. Locate and follow the
steps under Protocol Settings.
1. Click Describe Protocol and enter information about the
assay (optional).