Bio-Rad Bio-Plex Pro™ Rat Cytokine, Chemokine, and Growth Factor Assays - Manual (Page 13)

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4. Prepare Standards
General Instructions
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It is essential to reconstitute and dilute standards exactly as
described in this section. Incorrect preparation may lead to low signal,
high background, or inconsistent measurements from plate to plate
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The peel-off label provided with the standards lists the concentration
of the most concentrated dilution point, S1. Enter this information into
Bio-Plex Manager
™ software as instructed in Section 8
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For users who wish to mix assays from different panels, such as
diabetes assays with group I cytokines, guidance is provided here for
mixing 2 different lyophilized standards. Bead regions were chosen to
avoid overlap whenever possible. However, performance of multiplexes
containing assays from different groups have not been extensively
validated. Therefore, users must confirm that the assay performance is
still fit for purpose
Selecting a Diluent for Standards
Refer to Table 5 for recommended diluents based on different sample types.
As a general rule, reconstitute and dilute standards in a diluent similar to
the final sample type or sample matrix.
Table 5. Summary of recommended diluents for standards.
Sample Type
Diluent for Standards Add BSA
Serum and plasma Standard diluent None
Culture media, with serum Culture media None
Culture media, serum-free Culture media To 0.5% final
Lavage, sputum, other fluids Bio-Plex ® sample diluent To 0.5% final*
Lysate Bio-Plex sample diluent To 0.5% final*
* At least 0.5% final w/v BSA is recommended to stabilize analytes and reduce absorption
to labware.