Bio-Rad Sequi-Gen GT Sequencing Cell - Manual (Page 8)

The precision caster allows quick and easy gel casting without acrylamide spills or waste.
By casting the gel with a syringe through the precision caster base, gels can be poured in less
than 1 minute. The gel is cast with the glass plate assembly in the horizontal position. Two
full-length clamps secure the assembly and allow attachment of the precision caster base to
the bottom of the glass plate sandwich. A seal between the caster gasket and the plates is
created without tape or grease. The gel is injected from the bottom of the glass plate sandwich
(via the injection port of the precision caster base) and moves to the top of the glass plates as
a dome-shaped gel front. Acrylamide spills and waste can be eliminated by controlling the flow
of the gel front at the top of the glass plates.
Modular Assembly
There are four IPC dimensions to choose from, as shown in Figure 1.1. One universal
base functions as the lower buffer chamber for all IPC sizes.
Fig. 1.1. Interchangeable sizes.
1.2 Specifications
General Specifications
Base footprint 16 x 48 cm
Maximum unit height 65 cm (50 cm cells); 55 cm (40 cm cells); 45 cm (30 cm cells)
IPC sizes 21 x 40, 21 x 50, 38 x 30 cm and 38 x 50 cm
(width x length)
Actual gel sizes 17 x 40, 17 x 50, 34 x 30 cm, 34 x 50 cm
Gel thickness range 0.25 – 0.75 mm
Nominal gel volumes (0.25 mm ) 17 ml (21 x 40 cm); 21 ml (21 x 50 cm); 40 ml (38 x 30 cm);
43 ml (38 x 50 cm)
Nominal gel volumes (0.40 mm) 27 ml (21 x 40 cm); 34 ml (21 x 50 cm); 50 ml (38 x 30 cm);
68 ml (38 x 50 cm)
Minimum upper buffer volumes 500 ml (21 x 40 cm); 575 ml (21 x 50 cm); 650 ml
(38 x 30 cm); 1,400 ml (38 x 50 cm)
Minimum lower buffer volume 350 ml
Maximum lower buffer volume 500 ml
Electrical Specifications
Electrical Safety Certification IEC 1010-1
Rated voltage limit 3,000 volts
Rated power limit 100 watts
Rated temperature limit 60 ˚C
Electrical cables Rated to 3,000 volts (VDC)
Electrical leads Rated to 3,000 volts (VDC)
Banana plugs Rated to 3,000 volts (VDC)
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21 x 40 cm 21 x 50 cm 38 x 30 cm 38 x 50 cm